K562-PON[r] - RNA


K562-PON[r] - RNA

Cat. No.: 
Price $295.00
10 µg

Total RNA isolated from K562-PON[r] cells.

K562-PON[r] cells are 200-fold more resistant to PONATINIB than parental K562 cells: In vitro IC50s are 3.8 nM vs 0.02 nM, respectively.

Cell Line: K562 is an erythroleukemia cell line established from a 53 year old female with CML in blast crisis (Lozzio and Lozzio, 1975). The cell line bears the Philadelphia chromosome and associated BCR/ABL1 chimeric protein.

Parent phenotype: CD1-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9+, CD11b(+), CD13+, CD14-, CD15+ CD28-, CD10-, CD19-, CD20-, CD21-, CD22-, CD25, CD33+, CD34-, CD36-, CD38-, CD41+, CD42b-, CD44+, CD45+, CD56-, CD57-, CD61(+), CD65+, CD71+, CD80+, CD86-, CD117(+), CD122+, CD132+, CD235a/GlyA+, PPO-, vWF-, HLA-DR-, TdT-, EPO-R+, TCRα/β-, TCRγ/δ- (Drexler and Wehler).

Parent genotype: (COSMIC database, October 2014): Mutated genes: 197 point mutations, inserts and deletions reported. Key lesions are the BCR/ABL1 Fusion and CDK2NA mutation (c.151_457del307).

Compound: PONATINIB inhibits the tyrosine kinase domains of multiple proteins including ABL1, BCR/ABL1, RET, FGFR1-4, LCK and KIT.