K562-NIL[r] - RNA


K562-NIL[r] - RNA

Cat. No.: 
Price $295.00
10 µg

Total RNA derived from K562-NIL[r] cell line.

K562-NIL[r] cells are 15-fold more resistant to NILOTINIB than parental K562 cells: In vitro IC50s are 87 nM vs 5.9 nM, respectively.

Cell Line: K562 is an erythroleukemia cell line established from a 53 year old female with CML in blast crisis (Lozzio and Lozzio, 1975). The cell line bears the Philadelphia chromosome and associated BCR/ABL1 chimeric protein.

Parent phenotype: CD1-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9+, CD11b(+), CD13+, CD14-, CD15+ CD28-, CD10-, CD19-, CD20-, CD21-, CD22-, CD25, CD33+, CD34-, CD36-, CD38-, CD41+, CD42b-, CD44+, CD45+, CD56-, CD57-, CD61(+), CD65+, CD71+, CD80+, CD86-, CD117(+), CD122+, CD132+, CD235a/GlyA+, PPO-, vWF-, HLA-DR-, TdT-, EPO-R+, TCRα/β-, TCRγ/δ- (Drexler and Wehler).

Parent genotype: COSMIC database, October 2014): Mutated genes: 197 point mutations, inserts and deletions reported. Key lesions are the BCR/ABL1 Fusion and CDK2NA mutation (c.151_457del307).

Compound: NILOTINIB inhibits the tyrosine kinase domains in multiple proteins including ABL1, BCR/ABL1, KIT, LCK, EPHA3, EPHA8, DDR1, DDR2, PDGFRB, MAPK11 and ZAK.