K562-IMA[r]+PON[r] - CELLS


K562-IMA[r]+PON[r] - CELLS

Cat. No.: 
Price $995.00
1 million cells per cell line

K562-IMA[r]+PON[r] Viable Cells.

K562-IMA[r]+PON[r] cells are resistant to both IMATINIB and PONATINIB. Compared with parental K562 cells, K562-IMA[r]+PON[r] cells are 119-fold more resistant to IMATINIB and 4,255-fold more resistant to PONATINIB: The in vitro IC50s for K562-IMA[r]+PON[r] and parental K562 cells are 12.0 µm and 0.10 µm (IMATINIB), and 80 nM and 0.02 nM (PONATINIB), respectively.

K562-IMA[r]+PON[r] cells were established in two sequential steps. Step 1: K562-IMA[r] cells resistant to 2.0 µM IMATINIB were established by 8 months of continuous in vitro exposure of parental K562 cells to a progessively increasing concentration of IMATINIB; Step 2: These IMATINIB-resistant cells were maintained in 2.0 µM IMATINIB and then introduced and continuously exposed to a progressively increasing concentration of PONATINIB for an additional 3 months.

K562-IMA[r]+PON[r] cells are available through a licensing agreement with ChemoScreen. The listed price is for a two-year license.

Cell Line: K562 is an erythroleukemia cell line established from a 53 year old female with CML in blast crisis (Lozzio and Lozzio, 1975). The cell line bears the Philadelphia chromosome and associated BCR/ABL1 chimeric protein.

Parent phenotype: CD1-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9+, CD11b(+), CD13+, CD14-, CD15+ CD28-, CD10-, CD19-, CD20-, CD21-, CD22-, CD25, CD33+, CD34-, CD36-, CD38-, CD41+, CD42b-, CD44+, CD45+, CD56-, CD57-, CD61(+), CD65+, CD71+, CD80+, CD86-, CD117(+), CD122+, CD132+, CD235a/GlyA+, PPO-, vWF-, HLA-DR-, TdT-, EPO-R+, TCRα/β-, TCRγ/δ- (Drexler and Wehler).

Parent genotype: (COSMIC database, October 2014): Mutated genes: 197 point mutations, inserts and deletions reported. Most important are the BCR/ABL1 fusion and CDK2NA mutation (c.151_457del307).


IMATINIB inhibits the tyrosine kinase domains in multiple proteins including ABL1, BCR/ABL1, KIT and PDGF-R.

PONATINIB inhibits the tyrosine kinase domains in multiple proteins including ABL1, BCR/ABL1, RET, FGFR1-4, LCK and KIT.