K562-IMA[r]+DAS[r] - CELLS

Title

K562-IMA[r]+DAS[r] - CELLS

Cat. No.: 
10015C
Price $995.00
Unit: 
1 million cells per cell line
Description: 

K562-IMA[r]+DAS[r] Viable Cells.

K562-IMA[r]+DAS[r] cells are resistant to both IMATINIB and DASATINIB. Compared with parental K562 cells, K562-IMA[r]+DAS[r] cells are 129-fold more resistant to IMATINIB and 94-fold more resistant to IMATINIB: The in vitro IC50s for K562-IMA[r]+DAS[r] and parental K562 cells are 13.0 µm and 0.10 µm (IMATINIB), and 4.5 nM and 0.05 nM (DASATINIB), respectively.

K562-IMA[r]+DAS[r] cells were established in two sequential steps. Step 1: K562-IMA[r] cells resistant to 2.0 µM IMATINIB were established by 8 months of continuous in vitro exposure of parental K562 cells to a progessively increasing concentration of IMATINIB; Step 2: These IMATINIB-resistant cells were maintained in 2.0 µM IMATINIB and then introduced to, and continuously exposed to, a progressively increasing concentration of DASATINIB for an additional 3 months.

K562-IMA[r]+DAS[r] cells are available through a licensing agreement with ChemoScreen. The listed price is for a two-year license.

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Cell Line: K562 is an erythroleukemia cell line established from a 53 year old female with CML in blast crisis (Lozzio and Lozzio, 1975). The cell line bears the Philadelphia chromosome and associated BCR/ABL1 chimeric protein.

Parent phenotype: CD1-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9+, CD11b(+), CD13+, CD14-, CD15+ CD28-, CD10-, CD19-, CD20-, CD21-, CD22-, CD25, CD33+, CD34-, CD36-, CD38-, CD41+, CD42b-, CD44+, CD45+, CD56-, CD57-, CD61(+), CD65+, CD71+, CD80+, CD86-, CD117(+), CD122+, CD132+, CD235a/GlyA+, PPO-, vWF-, HLA-DR-, TdT-, EPO-R+, TCRα/β-, TCRγ/δ- (Drexler and Wehler).

Parent genotype: (COSMIC database, October 2014): Mutated genes: 197 point mutations, inserts and deletions reported. Most important are the BCR/ABL1 fusion and CDK2NA mutation (c.151_457del307).

Compounds:

IMATINIB inhibits the tyrosine kinase domains in multiple proteins including ABL1, BCR/ABL1, KIT and PDGF-R.

DASATINIB inhibits the tyrosine kinase domains in multiple proteins including including ABL1, BCR/ABL1, KIT, SRC, FYN, EPHB2, TYK2, FGR, BTK, TEC and DDR1.